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Sustained Gut Dysbiosis in Murine Polymicrobial Sepsis – Webinar Hosted by Shoreline Biome
October 25, 2019 @ 11:00 am - 12:00 pm
Sustained gut dysbiosis in murine polymicrobial sepsis:
Identification of longitudinal changes in the gut microbiome using strain-level resolution rRNA amplicon sequencing
Scientists don’t yet fully understand the relationship between sepsis and dementia, but sepsis can change the gut microbiome in ways that affect health and behavior. Candice Brown, an assistant professor at West Virginia University’s School of Medicine and Rockefeller Neuroscience Institute, is studying this relationship to discover how to prevent or mitigate the neurological impact of sepsis. She conducted strain-level microbial profiling using Shoreline Biome’s StrainID kit, and revealed that microbial communities change in response to sepsis.
Microbiome profiling of rRNA gene amplicons is a well-understood method that is rapid and inexpensive, but standard short-read 16S rRNA gene methods generally cannot differentiate between closely related strains. When Shoreline Complete StrainID amplicons are run on the PacBio Sequel, the combination of non-bead-beating lysis, comprehensive PCR primer pools, a 16S 23S amplicon product, and Shoreline Biome’s long-read Athena database can enable differentiation of closely related strains within and across samples, using a simple, rapid, and cost-effective approach.
You’ll learn:
How sepsis can change the gut microbiome in ways that affect health and behavior, and how strain-level microbial profiling reveals that the gut microbiome can change in response to sepsis.
Why the combination of non-bead-beating lysis, PCR design, long-read amplicon, and database are key to identifying microbial strains that influence human health.
Why Dr. Brown chose to use Shoreline Biome’s StrainID kit and how you can use it in your research for differentiation of closely related strains within and across samples, and with a simple, rapid, and cost-effective approach.
Presenters:
Candice Brown, Ph.D., Assistant Professor, Department of Neuroscience and Rockefeller Neuroscience Institute | West Virginia University School of Medicine
Mark Driscoll, Ph.D., Co-founder and Chief Scientific Officer, Shoreline Biome